Sahib, H., AlZubaidy, A., Jasim, G. (2016). Anti- Angiogenic Activity of Vitex agnus castus Methanol Extract in vivo Study. Iranian Journal of Pharmaceutical Sciences, 12(1), 59-68. doi: 10.22034/ijps.2016.19006
Hayder B Sahib; Adeeb A AlZubaidy; Ghaith A Jasim. "Anti- Angiogenic Activity of Vitex agnus castus Methanol Extract in vivo Study". Iranian Journal of Pharmaceutical Sciences, 12, 1, 2016, 59-68. doi: 10.22034/ijps.2016.19006
Sahib, H., AlZubaidy, A., Jasim, G. (2016). 'Anti- Angiogenic Activity of Vitex agnus castus Methanol Extract in vivo Study', Iranian Journal of Pharmaceutical Sciences, 12(1), pp. 59-68. doi: 10.22034/ijps.2016.19006
Sahib, H., AlZubaidy, A., Jasim, G. Anti- Angiogenic Activity of Vitex agnus castus Methanol Extract in vivo Study. Iranian Journal of Pharmaceutical Sciences, 2016; 12(1): 59-68. doi: 10.22034/ijps.2016.19006
Anti- Angiogenic Activity of Vitex agnus castus Methanol Extract in vivo Study
1Deputy Dean/ College of Pharmacy/ Al-Nahrain University/ Baghdad/ Iraq
2Dean college of pharmacy/ AlNahrain University
3Lecturer/ college of pharmacy/ al-Mustanseriya university
Abstract
This study aimed to investigate the anti-angiogenic activity of Vitex agnus castus methanol extract in vivo. Eggs were incubated for three days, small whole made on the fine pinpoint, next day the egg’s sac penetrated and a small frame was made in the shell. The window was resealed and eggs were returned to the incubator until day 10 of chick embryo development, 20 µl of 500mg/ml of the methanol extract transferred to the Chick Embryo Chorioallantoic Membrane (CAM), and eggs incubated for 72 hours (n = 6); The zone of inhibition calculated as mean of inhibition area in millimetre (mm) ±Standard deviation (SD). Functional groups of the chemicals component inside the extract has been identified by Fourier transform infrared spectroscopy FT-IR and High performance liquid chromatography HPLC used to identify the most likely causative agent. The results showed that the zones of inhibition area more than 10 mm. FT-IR showed that some of the identified functional group may relate to flavonoids. Casticin has identified in methanol extract. Because of the above results the mechanism of anti-angiogenic activity for the methanol extract of the Vitex agnus castus may relate to the Casticin which has the ability to block the VEGF-receptor, thereby inhibiting the angiogenesis process.
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