Voltage-Gated Sodium Channels Modulation by Bothutous Schach Scorpion Venom

Document Type: Research Paper

Authors

1 Student Research Committee

2 Department of Toxicology and Pharmacology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran

3 Department of physiology and pharmacology, Pasteur Institute of Iran, Tehran, Iran.

4 Department of Venomous Animals and Antivenom Production, Razi Vaccine and Serum Research Institute, Karaj, Iran.

Abstract

Buthotus schach is one of the dangers scorpion in Iran that belong to the Buthidae family. Toxins are existing in venom scorpion, modulate the ion channels by blocking or opening the pore of the channel or by altering the voltage gating and useful as pharmacological tools. In the present study, we investigated the effect of venom and its obtained fractions by gel filtrations on electrophysiological properties of magnocellular supraoptic of hypothalamus by using whole cell patch clamp. In our result were shown, scorpion crude venom and its fraction effect on Na gated voltage channels. A significant decrease was revealed in amplitude firing, in venom various concentration and some of the venom fraction. Also a significant increase was shown in half width and rise time 10% to 90% actions potential firing. Previous evidence was revealed change in electrophysiological properties such as amplitude and rise time 10% to 90%, related to sodium gated voltage channels. Sodium channels toxins existed on scorpion venom caused modulate on sodium channels. In order to access bioactive components, six fractions were collected by gel chromatography techniques. After bath application of fraction, four components were found F2, F3, F4 and F6. Also F2 and F3 component show the same effects than venom on electrophysiological properties of magnocellular supraoptic than venom.

Keywords

Main Subjects