Document Type : Research Paper
Biology Department, ecology and terrestrial and aquatic system laboratory, University Badji Mokhtar, BP 12, Sidi Ammar, 23000 Annaba, Algeria; Laboratory of Parasitic Eco-Epidemiology and populations genetics, Institut Pasteur of Algiers, Algiers, Algeria
Department of Biology of populations and organisms, LRZA laboratory, USTHB, Bp 32 El-Alia, Babezouar 16111 Algiers, Algeria
Department of Chemistry, DBA center, University of Lleida, AlcaldeRoviraRoure 191, 25198 Lleida, Spain; Scientific Technical Services TCEM, University of Lleida, Alcalde Rovira Roure 191, 25198 Lleida, Spain
Laboratory of Parasitic Eco-Epidemiology and populations genetics, Institut Pasteur of Algiers, Algiers, Algeria
Biology Department, ecology and terrestrial and aquatic system laboratory, University Badji Mokhtar, BP 12, Sidi Ammar, 23000 Annaba, Algeria
Department of Chemistry, DBA center, University of Lleida, AlcaldeRoviraRoure 191, 25198 Lleida, Spain
Traganum nudatum Delile is a North African endemic medicinal plant commonly used in traditional medicine to treat wounds, hemorrhoids, rheumatism and ear infections. Nevertheless, there are no scientific reports on the anti-inflammatory and analgesic effects of this plant. The present study aims to assess the therapeutic effect of the aqueous extract of the aerial part of the plant at three doses (40, 80 and 120 mg/kg) on experimentally pain-induced animals. First, the anti-inflammatory activity was assessed by carrageenan-induced paw edema on Swiss albino mice. Secondly, the analgesic activity was assessed by acetic acid-induced writhing test. In addition, UPLC-MS-PDA and GC-MS-FID analysis were performed to screen the possible therapeutic compounds. The anti-inflammatory effect of the aqueous extract at 120 mg/kg for 4 h experiment was significantly higher (89.97± 0.17%) than those of the reference drug acetylsalicylic acid (ASA) (0± 0.25%); while analgesic test showed a remarkable reducing pain effect (69.8 ± 1.7%) at the dose of 80 mg/kg, almost similar to those of Acetaminophen (Paracetamol) (72.54 ± 2.26 %). The phytochemical screening revealed the presence of therapeutic biomolecules such as flavonoids, mainly rutin (6440± 3.0 µg/g), flavonols (narcissoside 115.1± 1.4 µg/g) and phenolic acids (chlorogenic acid 1480± 1.6 µg/g). GC-MS-FID showed the presence of saturated acids such as behenic and palmitic acid with percentages of 35.58 ± 0.06 % and 17.54 ± 0.09 %, respectively, these were in higher percentage than the unsaturated fatty acids. These results validated the use of T.nudatum Delile for treating inflammatory and analgesic disorders in folk medicine. The presence of bioactive compounds including polyphenols and fatty acids may explain the pharmacological effect of the medicinal plant.