Document Type : Research Paper
Pharmacognosy Research Division, K L College of Pharmacy, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Guntur, Andhra Pradesh, India
Department of Pharmaceutical Analysis and Quality Control, Shri Vishnu College of Pharmacy, Bhimavaram, Andhra Pradesh, India
Department of Pharmaceutical chemistry, University College of Pharmaceutical Sciences, Visakhapatnam, India
Objective: To carry out an thorough study with a view to establish the therapeutic of methanolic and aqueous extract of root of Aralia racemosa and whole plant of Argyreia pilosa against Pheretima posthuma which is utilized as experimental model for helmintic.
Methods: The Aralia racemosa (Root), Argyreia pilosa (whole plant) and Indian adult earthworms (Pheretima posthuma) were collected and identified by approved taxonomist. Earthworms had been grouped and treated with extracts at concentration of 10, 20, 40 and 80mg/mL; albendazole of 40mg/mL as standard and normal saline as a control. The paralysis and death time was regarded as indicator of anthelmintic activity.
Results: Crude methanolic and aqueous extracts with concentrations of 10, 20, 40 and 80mg/ml of A. racemosa and A. pilosa showed concentration dependent activity but significant activity was observed at 80 mg/mL in both of the plants. The methanolic extract showed better activity than aqueous extracts at all concentrations. The methanolic extract of A. racemosa [P (min) = 2.01, D (min) = 10.52] and A. pilosa [P (min) = 2.45] respectively, the activity was found to be equivalent as compared to the standard drug Albendazole [P(min) = 2.28,D(min) = 5.44].
Conclusion: From the investigation, conclusion can be drawn that methanolic extracts showed better activity than aqueous extracts of A. racemosa and A. pilosa to treat intestinal worm infections. Since, this is a preliminary evaluation; isolation of chemical constituents which are responsible for the activity could be done in the future.