Evaluation of the Anti-tuberculosis and Cytotoxic Potential of the Seaweed Padina australis

Document Type: Research Paper

Authors

1 Applied Physiology Research Center, Isfahan University of Medical Sciences, Isfahan, Iran

2 Department of Pharmacognosy, School of Pharmacy and Pharmaceutical Sciences, and Isfahan Pharmaceutical Research Centre, Isfahan University of Medical Sciences Isfahan, Iran

3 Department of pharmacognosy, School of Pharmacy and Pharmaceutical Sciences and Isfahan Pharmaceutical Research Centre, Isfahan University of Medical Sciences Isfahan, Iran

4 Department of Pharmacognosy, Faculty of Pharmacy, Isfahan University of Medical Sciences

5 Applied Physiology Research Center, Cardiovascular research institute, Isfahan University of Medical Sciences Isfahan, Iran

10.22034/ijps.2018.90477.1463

Abstract

Background: Marine seaweeds synthesize different types of compounds with various biological activities, including anti-tuberculosis and anticancer effects.
Objective: The aim of this study was to evaluate anti tuberculosis of Padina australis and cytotoxicity of different fractions of this seaweed.
Methods: Padina australis was collected from coasts of Persian Gulf, identified and extracted by maceration with methanol-ethyl acetate. The extract was evaporated and partitioned by Kupchan method to yield Hexane, dichloromethane, Butanol and water partitions. The anti-tuberculosis activity of the crude extract and cytotoxicity of fractions were investigated using GFPMA and MTT methods.
Results: The cell survivals of HeLa cell were decreased by increasing the concentration of extracts. The IC50 value of hexane, dichloromethane, butanol and water partitions were 2.0, 20. , 19.7 and 182.7 μg/ml respectively. The crude extract was not active against tuberculosis.
Conclusion: This study reveals that different partitions of Padina australis have cytotoxic activity against cancer cell lines.

Keywords