Document Type: Research Paper
Department of Microbiology, Faculty of Medicine, Brawijaya University, Malang, East Java, Indonesia
Department of Pharmacy, Faculty of Medicine, Brawijaya University, Malang, East Java, Indonesia
Pharmacy Undergraduate Degree Program, Faculty of Medicine, Brawijaya University, Malang, East Java, Indonesia
Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis. The α-mangostin in mangosteen pericarp extract can inhibit M. tuberculosis growth. This study examined the potency of α-mangostin in mangosteen pericarp extract to inhibit 38-kDa and Ag85 protein secretion from M. tuberculosis H37Rv. The samples used in this study were divided into three independent variable groups (3.125 µg/ml; 6.25 µg/ml; and 12.5 µg/ml α-mangostin in the mangosteen pericarp extract), a positive control group (rifampin), a negative control group (M. tuberculosis H37Rv), and the Garcia® group (trademark of the mangosteen peel extract capsule). HPLC-MS/MS detected that the mangosteen pericarp extract contained 5,984.55 µg/g α-mangostin. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and dot blot analysis were conducted to analyze the profile and specificity of both proteins. This study revealed that 6.25 µg/ml of mangosteen pericarp extract had the greatest potency for inhibiting 38-kDa protein secretion from M. tuberculosis H37Rv, and 12.5 µg/ml mangosteen pericarp extract had greater potency for inhibiting Ag85 protein secretion than did the other doses. Thus, α-mangosteen from mangosteen pericarp extract can inhibit Ag38 and Ag85 secretion.