Role of Falcaria vulgaris on nicotine toxicity in rat hippocampus

Document Type : Research Paper

Authors

1 Department of Anatomical Sciences, Medical School, Kermanshah University of Medical Sciences, Kermanshah, Iran

2 Medical Biology Research Center, Department of Anatomical Sciences, Kermanshah University of Medical Sciences, Kermanshah, Iran

10.22034/ijps.2019.99222.1507

Abstract

Background: Falcaria vulgaris (F. vulgaris) is a vegetable that contains antioxidant ingredients. Nicotine is the most important alkaloid compound in tobacco and is a major risk factor in the development of functional disorder of several organ systems. Methods: In this study, 48 male rats were randomly assigned to 8 groups: Normal control (saline) group, nicotine control group (0.5 mg/kg), F. vulgaris groups (50, 100, and 150 mg/kg), and nicotine + F. vulgaris groups (10, 30, and 60 mg/kg). Treatments were administered intraperitoneally daily for 28 days. FRAP method was applied to determine the total antioxidant capacity. The number of dendritic spines was investigated by Golgi staining technique. Cresyl violet staining method was used to determine the number of neurons in hippocampal region CA1. Also, Griess technique was used to determine serum nitrite oxide level. Results: Nicotine administration increased significantly nitrite oxide level and total antioxidant capacity and a decreased number of neuronal dendritic spines and neurons compared to the normal control group (P < 0.05). In the F. vulgaris and nicotine + F. vulgaris groups, in all dosages, the number of neurons and neuronal dendritic spines increased significantly while nitrite oxide level and total antioxidant capacity decreased compared to the nicotine control group (p < 0.05). Conclusion: It seems that F. vulgaris administration improves hippocampal region CA1 injury in rates because of nicotine.

Keywords

References

[1] Salahshoor MR, Mohammadi MM, Roshankhah S, Najari N, Jalili C. Effect of Falcaria vulgaris on oxidative damage of liver in diabetic rats. J. Diabetes Metab. Disord. (2019): 1-9.
[2] Rafiey Z, Jalili F, Sohrabi M, Salahshoor MR, Jalili C. Effects of hydro-alcoholic extract of falcaria vulgaris on pancreas tissue in streptozotocin-induced diabetic rats. Iranian J. Endocrin Metab. (2017) 19 (2): 91-8.
[3] Hamideh J, Khosro P, Javad ND. 2012. Callus induction and plant regeneration from leaf explants of Falcaria vulgaris an important medicinal plant. J. Med. Plant Res. (2012) 6 (18): 3407-14.
[4] Jalili C, Kamani M, Roshankhah S, Sadeghi H, Salahshoor MR. Effect of Falcaria vulgaris extracts on sperm parameters in diabetic rats. Androl. (2018) 50 (10): e13130.
[5] Fazly Bazzaz BS, Haririzadeh G, Imami SA, Rashed MH. Survey of Iranian plants for alkaloids, flavonoids, saponins, and tannins [Khorasan Province]. Int. J. Pharm. (1997) 35 (1): 17-30.
[6] Esfandiari E, Roshankhah S, Mardani M, Hashemibeni B, Naghsh E, Kazemi M, Salahshoor MR. The effect of high frequency electric field on enhancement of chondrogenesis in human adipose-derived stem cells. Iran J. Basic Med. Sci. (2014) 17 (8): 571.
[7] Kozlowski LT, Mehta NY, Sweeney CT, Schwartz SS, Vogler GP, Jarvis MJ, West RJ. Filter ventilation and nicotine content of tobacco in cigarettes from Canada, the United Kingdom, and the United States. Tob. Control. (1998) 7 (4): 369-75.
[8] Filbey FM, Gohel S, Prashad S, Biswal BB. Differential associations of combined vs. isolated cannabis and nicotine on brain resting state networks. Brain Struct.  Func. (2018) 7: 1-0.
[9] Timmerman W, Westerink BH. Brain microdialysis of GABA and glutamate: what does it signify?. Synapse. (1997) 27(3): 242-61.
[10] Jalili C, Khani F, Salahshoor MR, Roshankhah SH. Protective effect of curcumin against nicotine-induced damage on reproductive parameters in male mice. Int. J. Morphol. (2014) 32 (3): 844-9.
[11] Decina P, Caracci G, Sandik R, Berman W, Mukherjee S, Scapicchio P. Cigarette smoking and neuroleptic-induced parkinsonism. Biol. Psychiatry. (1990) 28 (6): 502-8.
[12] Salahshoor MR, Mohamadian S, Kakabaraei S, Roshankhah Sh, Jalili C. Curcumin Improves liver Damage in Male Mice Exposed to Nicotine. J. Tradit. Complement Med. (2016) 176-183.
[13. Changeux JP. Nicotine addiction and nicotinic receptors: lessons from genetically modified mice. Nature Rev. Neurosci. (2010) 11 (6): 389.
[14] jalili. C, Salahshoor MR, Pourmotabbed A, Moradi S, Motaghi M, Shabanizadeh Darehdori A, Motaghi M. The Effects of Aqueous Extract of Boswellia Serrata on Hippocampal Region CA1 and learning deficit in kindled Rats. Res. Pharm. Sci. (2014) 9 (5): 351-358.
[15] Salahshoor MR, Khazaei M, Jalili C, Keivan M. Crocin improves damage induced by nicotine on a number of reproductive parameters in male mice. Int. J. Fertil. Steril. (2016) 10 (1): 71.
[16] Jalili C, Ahmadi S, Roshankhah S, Salahshoor M. Effect of Genistein on reproductive parameter and serum nitric oxide levels in morphine-treated mice. Int. J. Reprod. Biomed. (2016) 14(2): 95.
[17] Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Anal. Biochem. (1996) 239(1): 70-6.
[18] Huhn S, Beyer F, Zhang R, Lampe L, Grothe J, Kratzsch J, Willenberg A, et al. Effects of resveratrol on memory performance, hippocampus connectivity and microstructure in older adults–A randomized controlled trial. NeuroImage. (2018) 174: 177-90.
[19] Salahshoor MR, Roshankhah S, Hosseni P, Jalili C. Genistein Improves Liver Damage in Male Mice Exposed to Morphine. Chin. Med. J. (2018) 131(13): 1598-604.
[20] Oddo S, Caccamo A, Green KN, Liang K, Tran L, Chen Y, Leslie FM, et al. Chronic nicotine administration exacerbates tau pathology in a transgenic model of Alzheimer's disease. Proc. Natl. Acad. Sci. USA. (2005) 102 (8): 3046-51.
[21] Androuin A, Potier B, Nägerl UV, Cattaert D, Danglot L, Thierry M, Youssef I, et al. Evidence for altered dendritic spine compartmentalization in Alzheimer’s disease and functional effects in a mouse model. Acta. Neuropathol. (2018) 25: 1-6.
[22] Brown R. Nicotine sensitization increases dendritic length and spine density in the nucleus accumbens and cingulate cortex. Brain. Res. (2001) 899: 94-100.
[23] Blum R, Kafitz KW, Konnerth A. Neurotrophin-evoked depolarization requires the sodium channel Na V 1.9. Nature. (2002) 419(6908): 687.
[24] Oda A, Nakagomi S. Nicotine induces dendritic spine remodeling in cultured hippocampel neurons. J. Neurochem. (2014) 128: 246-55.
[25] Shin HJ, Son E, Lee DH, Kim HJ, Kang SS, Cho GJ, Choi WS, et al. Curcumin attenuate the Kainic acid Induced hippocampal cell death in mice. Neurosci. Lett. (2007) 416 (1): 49-54.
[26] Ying L, Hofseth LJ. An emerging role for endothelial nitric oxide synthase in chronic inflammation and cancer. Cancer Res. (2007) 67(4): 1407-10.
[27] Piri M, Nasehi M, Shahab Z, Zarrindast MR. The effects of nicotine on nitric oxide induced anxiogenic-like behaviors in the dorsal hippocampus. Neurosci. Lett. (2012) 528(2): 93-8.
[28] Keser A, Nesil T, Kanit L, Pogun S. Brain nitric oxide metabolites in rats preselected for nicotine preference and intake. Neurosci. Lett. (2013) 545: 102-6.
[29] Kahnamoei JR, Maleki F, Nasirzadeh M, Kishizadeh F. The Effects of Cigarette Smoking on Plasma MDA And TAC in University Students. Bull. Env. Pharmacol. Life Sci. (2014) 3:95-8.
[30] Nickander KK, Mcphee BR, Low PA, Tritschler H. Alpha-lipoic acid: antioxidant potency against lipid peroxidation of neural tissues in vitro and implications for diabetic neuropathy. Free Radic. Biol. Med. (1996) 21 (5): 631-9.
Iranian Journal of Pharmaceutical Sciences
Volume 16, Issue 3
July 2020
Pages 33-44

Files

Share

How to cite

Statistics