Document Type : Research Paper
Student research committee, Birjand University of medical sciences, Birjand, Iran
Cellular and molecular research center, Birjand University of medical sciences, Birjand, Iran
Department of chemistry, Faculty of science, University of Birjand, Birjand, Iran
Silymarin is a safe herbal medicine; however, it has some undesirable properties such as short half-life and poor aqueous solubility.
To the best of our knowledge, this study is the first to report utilizing a dual-drug delivery system (DDDS) to enhance the release profile of silymarin from both micelles and hydrogels.
In this experimental study, the release profile of micellar silymarin and micelle-hydrogel bounded silymarin during 21 days was examined using Knauer K2600A liquid chromatography.
The calibration curve was plotted using the peak-areas of the silymarin at different concentrations.
The RP-C18 column allowed a good separation of the components of standard silymarin.
LOD and LOQ were 16.5 and 55.02 μg/ml, respectively. The in vitro release profiles of the two compounds showed a rapid release of silymarin, especially in the absence of hydrogel. The cumulative release graph revealed that the hydrogel-bound form has more constant release kinetics than the free micelle form; this means that the hydrogel-bound form may sustain for longer durations.
In this study, a dual-drug delivery system based on hydrogel/micelle composites was introduced.
The results showed that Puramatrix hydrogel plays an important role in the constant release of silymarin. Furthermore, the RP-HPLC method presented in this study can be used by other researchers to overcome the difficulties associated with the in-vitro separation and quantification of silymarin.