The study of silymarin release kinetic in free and hydrogel bound micellar forms: a qualitative and quantitative analysis using RP-HPLC

Document Type: Research Paper


1 Student research committee, Birjand University of medical sciences, Birjand, Iran

2 Cellular and molecular research center, Birjand University of medical sciences, Birjand, Iran

3 Department of chemistry, Faculty of science, University of Birjand, Birjand, Iran



Silymarin is a safe herbal medicine; however, it has some undesirable properties such as short half-life and poor aqueous solubility.
To the best of our knowledge, this study is the first to report utilizing a dual-drug delivery system (DDDS) to enhance the release profile of silymarin from both micelles and hydrogels.
In this experimental study, the release profile of micellar silymarin and micelle-hydrogel bounded silymarin during 21 days was examined using Knauer K2600A liquid chromatography.
The calibration curve was plotted using the peak-areas of the silymarin at different concentrations.
The RP-C18 column allowed a good separation of the components of standard silymarin.
LOD and LOQ were 16.5 and 55.02 μg/ml, respectively. The in vitro release profiles of the two compounds showed a rapid release of silymarin, especially in the absence of hydrogel. The cumulative release graph revealed that the hydrogel-bound form has more constant release kinetics than the free micelle form; this means that the hydrogel-bound form may sustain for longer durations.
In this study, a dual-drug delivery system based on hydrogel/micelle composites was introduced.
The results showed that Puramatrix hydrogel plays an important role in the constant release of silymarin. Furthermore, the RP-HPLC method presented in this study can be used by other researchers to overcome the difficulties associated with the in-vitro separation and quantification of silymarin.