Profile of TLC-densitometry Annona squamosa and Persea americana as Antihyperlipidemic Medicinal Raw Material

Document Type : Research Paper

Author

Pharmacy Study Program, Faculty of Health Sciences, Muhammadiyah University of Malang, Malang, East Java, Indonesia

10.22034/ijps.2020.121834.1643

Abstract

Natural medicine products usually consist of two to five kinds of plants that have synergistic biological activity. The responsible constituents for the therapeutic activity in plants often involve diverse chemical components. The combination of ethanol extracts of Annona squamosa L. (EEAS) and Persea americana M. (EEPA) has been shown to have antihyperlipidemic activity. To ensure the correctness and consistency of the EEAS and EEPAs combination as the antihyperlipidemic treatments, it is necessary to characterize the plant’s secondary metabolites. This study aimed to obtain the profiles of the secondary metabolites of Annona squamosa L. and Persea americana M. using the TLC-densitometry technique. Extract samples were applied to TLC plates and screened using densitometry at 254 nm. The obtained chromatogram peak was measured at the wavelength range of 200–700 nm to obtain the maximum wavelength of each peak. The results showed that EEAS had 5 peaks with rf values of 0.00, 0.61, 0.67, 0.73, and 0.87. The TLC-densitometry analysis results of EEPA showed eight peaks with rf values of 0.00, 0.11, 0.19, 0.49, 0.56, 0.71, 0.77, and 0.90. The extract chromatograms spectra showed that 2–3 peaks at the wavelength range of 300-350 nm. Chromatogram TLC-densitometry profile data on EEAS and EEPA can be used as the indicators in the raw material combinations standardization process of EEAS and EEPA as natural medicine products to treat antihyperlipidemic.

Keywords

References

[1] Mahomoodally MF. Traditional Medicines in Africa : an Appraisal of Ten Potent African Medicinal Plants. Evid Based Complement Alternat. Med. (2013) 2013: 617459.
[2] Bauer R. Quality criteria and standardization of phytopharmaceuticals: Can acceptable drug standards be achieved?. Ther. Innov. Regul. Sci. (2013) 32:101–10.
[3] Werner B. The Significance of Quality for Efficacy and Safety of Herbal Medicinal Products. Drug. Inf. J. (2000) 34:15–23.
[4] Rofida S, Firdiansyah A and Fitriyastuti E. Aktivitas Antihiperlipidemia Ekstrak Etanol Antihyperlipidemic Activity of Annona squamosa L. Leaves Ethanolic Extract. J. Pharm. Sci. Pharm. Pract. (2015) 2(1):1–3.
[5] Gajalakshmi S, Divya R, Deepika VD, Mythili S and Sathiavelu A. Pharmacological Activities of Annona Squamosa: A Review. Int. J. Pharm. Sci. Rev. Res. (2011) 10(2): 24–9.
[6] Saha R. Pharmacognosy and pharmacology of Annona squamosa: A review. Int. J. Pharm. Life Sci. (2011) 2(10): 1183–9.
[7] Handayani S, Saryono and Herayanti. Effek Daun Alpukat (Persea americana M.) dan Daun Kelor (Moringa oleifera L.) Terhadap Peningkatan Kadar HDL pada Model Tikus Putih Hiperlipidemia. J. Kep. Soedirman (The Soedirman J. of Nurs.). (2017) 12: 47-55.
[8] Nathaniel OB, Selina AS, John KM, Mercy B, Sylvester AA and Michael B. Phytoconstituents, Antimicrobial and antioxidant properties of the leaves of Persea americana Mill cultivated in Ghana. J. Med. Plants Res. (2016) 9(36): 933–939.
[9] Tabeshpour J, Razavi BM and Hosseinzadeh H. Effects of Avocado (Persea americana) on Metabolic Syndrome: a Comprehensive Systematic Review. Phytother. Res. (2017) 31(6): 819-837.
[10] Haryoto, Suhendi A and Ahwan. Identification and Antioxidant Activity of Non Polar Fraction Annona squamosa L. With DPPH Assay. Pharmacon. (2009) 10: 51-56.
[11] Pandey N and Barve D. Phytochemical and Pharmacological Review on Annona squamosa Linn. Int. J. Res. Pharm. Biomed. Sci. (2011a) 2(4): 1404–12.
[12] Pandey N, Barve D, Pharmacy TITCO, Nagar A and Bhopal MP. Review Paper Phytochemical and Pharmacological Review on Annona squamosa Linn. Int. J. Res. Pharm. Biomed. Sci. (2011b) 2(4): 1404–12.
[13] Bhakuni DS, Tewari S and Dhar MM. Aporphine Alkaloids of Annona Squamosa. Phytochemist. (1972) 11: 18–22.
[14] Chang FR, Chen CY, Hsieh TJ, Cho CP and Wu YC. Chemical constituents from Annona glabra III. J. Chinese Chem. Soc. (2000) 47(4B): 913–20.
[15] Fu D, Yu JY, Connell AR, Yang S, Hookham MB, McLeese R and Lyons TJ. Beneficial effects of berberine on oxidized LDL-induced cytotoxicity to human retinal müller cells. Invest. Ophthalmol. Vis. Sci. (2016) 57: 3369–79.
[16] Xu RX, Sun XC, Ma CY, Yao YH, Li XL, Guo YL, Zhang Y, Li S and Li JJ. Impacts of berberine on oxidized LDL-induced proliferation of human umbilical vein endothelial cells. Am. J. Transl. Res. (2017) 9 (10): 4375–89.
[17] Owolabi MA, Coker HAB and Jaja SI. Bioactivity of the phytoconstituents of the leaves of Persea americana. J. Med. Plants. Res. (2010) 4(12): 1130–5.
[18] Fuhrman B and Aviram M. Flavonoids protect LDL from oxidation and attenuate atherosclerosis. Curr. Opin. Lipidol. (2001) 12 (1): 41–8.
Iranian Journal of Pharmaceutical Sciences
Volume 16, Issue 4
October 2020
Pages 1-8

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