Document Type : Research Paper
Authors
1
Department of Pharmacognosy School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
2
Department of Pharmaceutical Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
3
Department of Pharmaceutical Biotechnology, School of Pharmacy; Mashhad University of Medical Sciences, Mashhad, Iran
4
Department of Pharmacognosy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
5
Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
Abstract
Nepeta glomerulosa Boiss. is a medicinal plant has been used in traditional medicine. The aim of this study was to evaluate the anti-melanogenesis inhibitory activity of MeOH, n-hexane, ethyl Acetate (EtAc), dichloromethane (CH2Cl2), n-butanol (BuOH) and H2O extracts isolated from N. glomerulosa in B16 melanoma cell line. The B16F10 cell line viability after treatment with increasing concentrations of different extracts of the plant (50-200 µg/ml) was measured by using MTT, the inhibitory effect on synthesis of melanin, mushroom tyrosinase activity, cellular tyrosinase and effect on oxidative stress was determined by the colorimetric and fluorometric method. The data showed that at concentrations our results showed the melanogenesis inhibitory and antioxidant effects of N. glomerulosa on B16F10 cells may recommend a novel agent in diminishing skin pigmentation and skin aging in cosmetic industry.
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