Evaluation of the Anti-tuberculosis and Cytotoxic Potential of the Seaweed Padina australis

Document Type : Research Paper

Authors

1 Applied Physiology Research Center, Isfahan University of Medical Sciences, Isfahan, Iran

2 Department of Pharmacognosy, School of Pharmacy and Pharmaceutical Sciences, and Isfahan Pharmaceutical Research Centre, Isfahan University of Medical Sciences Isfahan, Iran

3 Department of pharmacognosy, School of Pharmacy and Pharmaceutical Sciences and Isfahan Pharmaceutical Research Centre, Isfahan University of Medical Sciences Isfahan, Iran

4 Department of Pharmacognosy, Faculty of Pharmacy, Isfahan University of Medical Sciences

5 Applied Physiology Research Center, Cardiovascular research institute, Isfahan University of Medical Sciences Isfahan, Iran

10.22034/ijps.2018.90477.1463

Abstract

Background: Marine seaweeds synthesize different types of compounds with various biological activities, including anti-tuberculosis and anticancer effects.
Objective: The aim of this study was to evaluate anti tuberculosis of Padina australis and cytotoxicity of different fractions of this seaweed.
Methods: Padina australis was collected from coasts of Persian Gulf, identified and extracted by maceration with methanol-ethyl acetate. The extract was evaporated and partitioned by Kupchan method to yield Hexane, dichloromethane, Butanol and water partitions. The anti-tuberculosis activity of the crude extract and cytotoxicity of fractions were investigated using GFPMA and MTT methods.
Results: The cell survivals of HeLa cell were decreased by increasing the concentration of extracts. The IC50 value of hexane, dichloromethane, butanol and water partitions were 2.0, 20. , 19.7 and 182.7 μg/ml respectively. The crude extract was not active against tuberculosis.
Conclusion: This study reveals that different partitions of Padina australis have cytotoxic activity against cancer cell lines.

Keywords

References

[1] Mdluli K, Slayden RA, Zhu Y, Ramaswamy S, Pan X, Mead D, et al. Inhibition of a Mycobacterium tuberculosis β-ketoacyl ACP synthase by isoniazid. Science (1998) 280: 1607-1610.
[2] Gulland, A. Global cancer prevalence is growing at alarming pace, says WHO. Brit. Med. J. (2014) 348: 1338.
[3] Smit AJ. Medicinal and pharmaceutical uses of seaweed natural products: a review. J. Appl. Phycol. (2004) 16(4): 245-262.
[4] Blunt JW, Copp BR, Hu W, Munro M, Northcote PT and Prinsep MR. Marine natural products. Nat. Prod. Rep. (2007) 24(1): 31-86.
[5] Yegdaneh A, Putchakarn S, Yuenyongsawad S, Ghannadi A and Plubrukarn A. 3-oxoabolene and 1-oxocurcuphenol, aromatic bisabolanes from the sponge Myrmekioderma sp. Nat. Prod. Commun. (2013) 8(10): 1355-1357.
[6] Kuda T, Taniguchi E, Nishizawa M and Araki Y. Fate of water-soluble polysaccharides in dried Chorda filum a brown alga during water washing. J. Food. Compost. Anal. (2002) 15(1): 3-9.
[7] Garg HS, Sharma M, Bhakuni DS, Pramanik BN and Bose AK. An antiviral sphingosine derivative from the green alga Ulva fasciata. Tetrahedron. Lett. (1992) 33(12): 1641-1644.
[8] TÜney İ., Cadirci BH, Ünal D and Sukatar A. Antimicrobial activities of the extracts of marine algae from the coast of Urla (Izmir, Turkey). Turk. J. Biol. (2006)30(3): 171-175.
[9] Ghannadi A, Zandi K, Sartavi K and Yegdaneh A. Screening for antimalarial and acetylcholinesterase inhibitory activities of some Iranian seaweeds. Res. Pharm. Sci. (2012) 8(2): 113-118.
[10] Mehdinezhad N, Ghannadi A and Yegdaneh A. Phytochemical and biological evaluation of some Sargassum species from Persian Gulf. Res. Pharm. Sci. (2016) 11(3): 243-249.
[11] Trono GC. Field guide and atlas of the seaweed resources of the Philippines, Bookmark. (1997)
[12] Yamamoto I, Takahashi M, Tamura E, Maruyama H and Mori H. Antitumor activity of edible marine algae: Effect of crude fucoidan fractions prepared from edible brown seaweeds against L-1210 leukemia. Eleventh International Seaweed Symposium, Springer. (1984)
[13] Xu N, Fan X, Yan X and Tseng C. Screening marine algae from China for their antitumor activities. J. Appl. Phycol. (2004)16(6): 451-456.
[14] Yegdaneh A, Ghannadi A and Dayani L. Chemical constituents and biological activities of two Iranian Cystoseira species. Res. Pharm. Sci. (2016) 11(4): 311.
[15] Khafaji A, El-Nakkadi A and El-Hassan A. Biological and biochemical studies on some components of Red Sea algae, possibility of using the plants as part of nutritional diets. Mar. Sci. (1992) 3(1).
[16] Sohrabipour J, Nejadsatari T, Assadi M and Rabei R. The marine algae of the southern coast of Iran, Persian Gulf, Lengeh area. Iran. J. Bot. (2004) 10(2): 83-93.
[17] Cooksey R, Crawford J, Jacobs W and Shinnick T. A rapid method for screening antimicrobial agents for activities against a strain of Mycobacterium tuberculosis expressing firefly luciferase. Antimicrob. Agents. Chemother. (1993) 37(6): 1348-1352.
[18] Nakhjavani M, Stewart DJ, Shirazi FH. Effect of steroid and serum starvation on a human breast cancer adenocarcinoma cell line. J. Exp. Ther. Oncol.( 2017) 1;12(1).
[19] Stanojković TP, Scaron K, Zdunić G, Kljajić Z, Grozdanić N and Antić J. In vitro antitumoral activities of Padina pavonia on human cervix and breast cancer cell lines. J. Med. Plants. Res. (2013) 7(8): 419-424.
[20]Guedes ÉA, Silva TG, Aguiar JS, Barros LD, Pinotti LM and Sant'Ana AE. Cytotoxic activity of marine algae against cancerous cells. Rev. Bras. Farmacogn. (2013) 23(4): 668-673.
[21] Mosaddegh M, Gharanjik B, Naghibi B, Esmaeili S, Pirani A, Eslami Tehrani B, et al. A survey of cytotoxic effects of some marine algae in the Chabahar coast of Oman Sea. Res. J. Pharmacogn. (2014) 1(1): 27-31.
[22] Yamamoto I, Takahashi M, Tamura E, Maruyama H and Mori H. Antitumor activity of edible marine algae: Effect of crude fucoidan fractions prepared from edible brown seaweeds against L-1210 leukemia. Eleventh International Seaweed Symposium, Springer. (1984)
[23] Kamenarska Z, Gasic M, Zlatovic M, Rasovic A, Kljajic Z, Stefanov K, et al. Chemical composition of the brown alga Padina pavonia (L.) Gaill. from the Adriatic Sea. Bot. Mar. (2002)45(4): 339-345.
[24] Coombe DR, Parish CR, Ramshaw IA and Snowden JM. Analysis of the inhibition of tumour metastasis by sulphated polysaccharides. Intl J Cancer (1987) 39(1): 82-88.
[25] Khanavi M, Nabavi M, Sadati N, Shams Ardekani M, Sohrabipour J, Nabavi SMB,et al. Cytotoxic activity of some marine brown algae against cancer cell lines. Biol. Res. (2010) 43(1): 31-37.
[26] Awad NE, Motawe HM, Selim MA and Matloub AA. Antitumourigenic polysaccharides isolated from the brown algae, Padina pavonia (L.) Gaill. and Hydroclathrus clathratus (C. Agardh) Howe. Med. Aromat. Plant. Sci. Biotechnol. (2009) 3: 6-11.
[27] Ktari L. and Guyot M. A cytotoxic oxysterol from the marine alga Padina pavonica (L.) Thivy. J appl. Phycol. (1999) 11(6): 511-513.
[28] Snell N. The treatment of tuberculosis: current status and future prospects. Expert. Opin. Investig. Drugs. (1998) 7(4): 545-552.
Iranian Journal of Pharmaceutical Sciences
Volume 15, Issue 1
January 2019
Pages 29-38

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