Screening methanolic extracts of Sutherlandia spp as anti-tumor agents and their effects on anti-apoptotic genes.

Document Type : Research Paper


University of Johannesburg, faculty of Science, Department of Biochemistry, Aucland Park Campus, Auckland Park, Johannesburg, South Africa.



Introduction: In South Africa, cervical cancer is the utmost common cancer in women with an annual crude incidence rate of 30.2 per 100,000 women. Many homes are still dependent on traditional medicine. Because of the name “cancer bush’ given to Sutherlandia frutescens (SF) plant by the traditional healers for its anti-cancer activity, the plant was used lines. Methods: MTT and xCELLigence were used to predict the IC50 and evaluate methanolic extract effect on SiHa cell lines. A flow cytometer was used to characterize the cell death type. Caspase 3/7 and ATP assays. Results: MTT and xCELLigence results showed a growth inhibition following treatment with varying concentrations of Sutherlandia frutescens with IC50 optimal at 50ug/ml. ATP level increased in S.F treated cells. Flow cytometry showed cells dying by apoptosis and with many cells trapped in S-Phase. Caspase activity was high in methanolic treated cells. The compounds used were: Canavanine, GABA and Pinitol. These compounds induced cell death at the highest concentration used. Conclusion: Sutherlandia frutescens has shown potential to disrupt DNA replication thereby damaging DNA and arresting cells at S-phase leading to cell death by apoptosis.


[1] Liu Y, Cherry JJ, Dineen JV, Androphy EJ, Baleja JD. Determinants of stability for the E6 protein of papillomavirus type 16. J. Mol. Biol. (2009) 386 (4): 1123-37.
[2] Van Schalkwyk SL, Maree JE, Wright SCD. Cervical cancer: the route from signs and symptoms to treatment in South Africa. Reprod. Heal. Mat. (2008) 16 (32): 9–17.
[3] Somdyala NIM, Bradshaw D, Curtis B, Gelderblom WCA. Cancer incidence in selected municipalities of the Eastern Cape Province, 1998-2002: PROMEC Cancer Registry Technical Report. Cape Town: South African Medical Research Council (2007).
[4] Talip QTheron GSteyn WHall D. Total perinatally related losses at Tygerberg Hospital - a comparison between 1986, 1993 and 2006. S. Afr. Med. J. (2010)100 (4): 250-3.
[5] Van Wyk BE, Albrecht C. A review of the taxonomy, ethnobotany, chemistry and pharmacology of Sutherlandia frutescens. J. Ethnopharmacol. (2008)119: 620-629.
[6] Moshe D, Bank HV, Bank MV, Van Wyk BE. Lack of genetic differentiation between 19 populations from seven taxa the sutherlandia Tribe. Biochem. Syst. Ecol. (1998) 26: 595–609.
[7] Mncwangi, Viljoen. Devil’s Claw—A review of the ethnobotany, phytochemistry and biological activity of Harpagophytumprocumbens. J. EthnoPharmacol. (2012) 143: 755-771.
[8] Newman DJCragg GM. Natural products as sources of new drugs over the 30 years from 1981 to 2010. J. Nat. Prod. (2012) 75(3):311-35.
[9] Gurib-Fakim A. Medicinal plants: traditions of yesterday and drugs of tomorrow. Mol. Aspects Med. (2006) 27(1):1-93.
[10] Rowinsky EKOnetto NCanetta RMArbuck SG. Taxol: the first of the taxanes, an important new class of antitumor agents. Semin. Oncol. (1992) 19 (6): 646-62.
[11] Legarza KYang LX. Novel camptothecin derivatives. In Vivo. (2005) 19 (1): 283-92.
[12] Choene M, Motadi LR. Validation of the Antiproliferative Effects of Euphorbia tirucalli Extracts in Breast Cancer Cell Lines. MOL. BIOL. (2016) 50 (1): 115-127.
[13] Cheone M, Motadi LR. Anti-Proliferative effects of methanolic extract of K. Foetidissima in breast cancer cell lines MCF7 and YMB1. Mol. Biol. (2012) 102.
[14] Thafeni MASayed YMotadi LR. Euphorbia mauritanica and Kedrostis hirtella extracts can induce anti-proliferative activities in lung cancer cells. Mol. Biol. Rep. (2012) 39 (12): 10785-94.
[15] Blázquez CCarracedo ABarrado LReal PJFernández-Luna JLVelasco GMalumbres MGuzmán M. Cannabinoid receptors as novel targets for the treatment of melanoma. FASEB. J. (2006) 20 (14): 2633-5.  
[16] Marcu JPChristian RTLau DZielinski AJHorowitz MPLee JPakdel AAllison JLimbad CMoore DHYount GLDesprez PYMcAllister SD. Cannabidiol enhances the inhibitory effects of delta9-tetrahydrocannabinol on human glioblastoma cell proliferation and survival. Mol. Cancer Ther. (2010) 9 (1): 180-9.
[17] Karasawa KMatsumoto FIto SOba SFuruya THirowatari HIzawa HIto KSasai K. Hyperfractionated radiotherapy with concurrent docetaxel for advanced head and neck cancer: a phase II study. Anticancer Res. (2012) 32(9): 4013-8.
[18] Mthembu NN,Motadi LR. Apoptotic potential role of Agave palmeri and Tulbaghia violacea extracts in cervical cancer cells. Molecular Biology Reports. Mol. Biol. Rep. (2014) 41(9): 6143-55.
[19] Tan DSKaye S. Ovarian clear cell adenocarcinoma: a continuing enigma. J. Clin. Pathol. (2008) 60 (4): 355-60.
[20] Tsujimoto Y. Apoptosis and necrosis: intracellular ATP level as a determinant for cell death modes. Cell Death Differ. (1997) 4(6): 429-34.
[21] Sakagami HSatoh KHakeda YKumegawa M. Apoptosis-inducing activity of vitamin C and vitamin K.  Mol. Cell Biol. (2000) 46(1):129-43.
[22] Matsumoto KOkano JNagahara TMurawaki Y. Apoptosis of liver cancer cells by vitamin K2 and enhancement by MEK inhibition. Int. J. Oncol. (2006) 29 (6): 1501-8.
[23] Yokoyama TMiyazawa KNaito MToyotake JTauchi TItoh MYuo AHayashi YGeorgescu MMKondo YKondo SOhyashiki K. Vitamin K2 induces autophagy and apoptosis simultaneously in leukemia cells. Autophagy. (2008) 4(5): 629-40.