Effect of Solvent Polarity on the Extraction Yield of Antioxidants from Lactobacillus Supernatants

Document Type : Research Paper


1 Molecular Biology Unit, Pasteur Institute of Iran, Pasteur Ave.,13164

2 Department of Microbiology, faculty of Biological Sciences, Alzahra University



There is increasing evidence to suggest that the Lactobacilli species possess antioxidant activities, however, there
are a few reports of optimization of solvent systems for the separation of their antioxidant compounds by thin-layer
chromatography. In the current study, we explore the efficiency of four organic solvents (aqueous, methanol, ethyl
acetate, and n-hexane) for the extraction of antioxidant materials from Lactobacillus supernatants. According to the
results, methanol extraction significantly increased the antioxidant properties of Lactobacillus supernatants. In
addition, the methanol extract of Lactobacillus supernatants was fractionated using thin-layer chromatography
(TLC). A solvent system consisting of methanol/chloroform is a promising approach for TLC analysis of
Lactobacillus supernatant. The partial purification of the antioxidant components using thin-layer chromatography
demonstrated a drastic rise in the antibacterial and antioxidant properties in comparison to the crude methanol extract
of the same sample. In this study, the TLC fractionation of Lactobacillus extracellular materials was described for
the first time. Further isolation and purification are essential to identify these bioactive compounds.